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3 Progress of Joint Research Activities
rd

Research No.: ② A-2 ODA Loan Joint Research Date: Oct. 26 , 2020
th
1 General Title Application of genetic, breeding and cell culture technologies
for new varieties of rice and other crops with improved
quality and adaptability to climate changes and other
environmental stresses

2 Core Members CTU: Huynh KY (Leader), Nguyen Loc Hien, Nguyen Chau Thanh Tung, Tran Thi Thanh Thuy (Ms), Le Vinh Thuc,
Nguyen Thanh Hoi, Ngo Phuong Ngoc (Ms), Nguyen Minh Chon, Tran Huu Phuc, Nguyen Thanh Tam
Japanese Universities: KISHIMA Yuji (Hokkaido University), YAMADA Tetsuya (TUAT)

3 Duration May 2017 – April 2020 (3 years) <Kick-off>
4 Main To improve productivity, quality, and adaptability of major crops under climate change
Objectives condition using genetic engineering, breeding and cell culture
5 Activities 1. Screening salt tolerance local varieties and identifying SNPs markers that correlate to salt stress

Control Treatment with 100 mM

Heat map of phenotypic
Screening salt tolerance local rices characteristics of local rices
2. Selected salt tolerance rice varieties for study the transcriptome and metabolite under salt
stress condition

Differences of ABA concentration in salt stress condition
18
16
14 12
ABA concentration (ug/uL) 10 8 6
2 4
0
Salt stress treatment for transcriptome and Metabolite analysis
S: Shoot; R: Root
3. Development of salt tolerance rice varieties using genetic engineering
Germination 2 weeks Sub-culture 5 days
on N6D on N6D Sub-culture on
MSNK
Editing candidated genes for
KD18
9 days salt tolerance trait using
CRISP/CAS9?

Sub-culture Sub-culture Sub-culture on
regeneration
on MS 2 weeks on MS 2 weeks medium MS +1B Expected results
Challenging:
6 Comments - The facility for transgenic plant is not ready yet.

ⓒ All Rights Reserved, Nov. 2020 (CTU)

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