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3 Progress of Joint Research Activities
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Research No.: ➅ A-6 ODA Loan Joint Research Date: Oct. 31 , 2020
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Studies on insect pests and diseases and development of
1 General Title
alternative plant protection technologies
CTU: Le Van Vang (Project Leader), Duong Kieu HANH (Ms.), Trinh Thi XUAN (Ms.), Nguyen Thi Thu NGA (Ms.),
2 Core Doan Thi Kieu TIEN (Ms.), Le Phuoc THANH, Pham Kim SON, Lang Canh PHU, Nguyen Chi CUONG
Members Japanese Universities: KAMEI Kaeko (KIT), ARIE Tsutomu (TUAT), MORIYAMA Hiromitsu (TUAT),
NAKAI Madoka (TUAT), INOUE Maki (TUAT), ANDO Tetsu (TUAT), YAMAMOTO Masanobu (TUAT)
3 Duration Oct. 2018 – Sep. 2021 (3 years) <2 Batch>
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To enhance the capacity in research and application of environmentally friendly strategies for
4 Main management of insect pests in the Mekong Delta of Viet Nam, from which provides the best means of
Objectives sustainable and integrated plant protection strategies, and thus elevates the competitiveness for
agricultural products on national and international markets
5 Focal Points The cabbage webworm, Hellula undalis Fab. (Lepidoptera: Crambidae: Glaphyriinae), is one of the world’s most
serious defoliators of cruciferous vegetables, especially head cabbage.
In order to enhance the attraction of the synthetic lure and to utilize it as a tool for preventing adult intrusion
and monitoring population dynamics in a sustainable management program in Viet Nam, the sex pheromone of H.
undalis was re-examined from the strain inhabiting the Mekong Delta using GC-EAD and GC-MS analyses and by
assessing the attraction of synthetic pheromone lures in fields.

This study is funded
in part by the CTU
Improvement GC-EAD and GC-MS
Project VN14-P6, analyses of the
supported by a pheromone gland and
Japanese ODA loan body extracts elucidated
Fig. 1. GC-EAD analyses of a Hellula undalis (Z)-11-tetradecenyl
female abdominal tip extract (1 FE) (A) and acetate (Z11-14:OAc)
synthetic standards (3 – 10 ng) (B) and (Z)-11-hexadecenal
(Z11-16:Ald), E11,E13-
16:Ald. the unsaturated
Table 1. Field attraction of H. undalis males by hydrocarbon (3Z,6Z,9Z)-
synthetic lures. 3,6,9-tricosatriene
(Z3,Z6,Z9-23:H) as
pheromone components
of H. undalis.
Fig. 2. GC-MS analysis of a Hellula undalis abdominal tip extract
(20 FE, upper) and crude body extract (9 FE, lower)

Field examinations indicated the significant role of
E11,E13-16:Ald as a major component and a synergistic
effect of the three new compounds as a minor
component. In that, the 3:3:7:1 was the best ratio
mixture for attraction of H. undalis males in field.
Pheromone lures will use as tool for understanding the relationship between the frequency of damaged green
mustard plants and the number of males caught in pheromone traps, along with the season of H. undalis flight in
6 Perspectives the Mekong Delta of Vietnam. In addition, we will try to find out whether this lure can attract males outside of the
Mekong Delta, especially in Japan
ⓒ All Rights Reserved, Nov. 2020 (CTU)

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