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3 Progress of Joint Research Activities
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                 Research No.:  ㉒㉒ F-6  ODA Loan Joint Research                            Date: Oct. 8 , 2020
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                1   General Title  Biochemistry and pharmaceutical science in aquaculture and fisheries
                                 Can Tho University: Tran Thi Tuyet Hoa (Leader), Pham Minh Duc, Bui Thi Bich Hang, Nguyen Thi Thu Hang,
                                 Dang Thuy Mai Thy, Huynh Truong Giang, Tran Thi My Duyen, Nguyen Trong Tuan, Dai Thi Xuan Trang, Le Thanh
               2   Core Members  Phuoc, Nguyen Phuc Dam, Ngo Quoc Luan, Luu Thai Danh, Doan Van Hong Thien
                                 Japanese Universities: KAMEI Kaeko (KIT), KANAORI Kenji (KIT)
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               3     Duration                       Jan. 2018 – Dec. 2020 (3 years)  <1 Batch>
                                 Develop natural bioactive compounds in order to:
               4  Main Objectives  1.Enhance of fish/ shrimp immune responses
                                 2.Replace antibiotics/ chemicals in the prevention and treatment of aquatic animal diseases
                5   Focal Points
                                      1. Screening bioactive compounds to apply in drug production for the
                                             prevention and treatment of aquatic animal diseases

                                  Plant extracts                                          45 plant extracts
                                  preparation                                             were identified
                                             Plant collection  Powdered plant  Mixture  Evaporation  Crude extracts
                                    In vitro                                    6; 15; 14 and 6 plant extracts showed
                                                                                 h hiigghh  aannttiimmiiccrroobbiiaall  aaccttiivviittyy against 4
                                  antimicrobial                                aquatic animal pathogens including A.
                                     tests                                      hydrophila, E. ictaluri, S. agalactiae
                                              Paper disc   Impregnated   Spreading   Placing   Interpreting   and V. parahaemolyticus, respectively
                                              preparation  paper disc  bacteria  impregnated disc  result
                                  Chemical analysis                                   11/20 pure compounds
                                  and identification                                      were identified
                                                  Crude   Extracts showed high   Chemical   Component
                                                  extracts  antimicrobial activity  analysis  identification
                                                        In vitro
                                                    antimicrobial tests       Acute toxicity test
                                    2 2..  AAsssseessssiinngg  tthhee  ppoossiittiivvee  iimmppaacctt  ooff  nnaattuurraall  bbiiooaaccttiivvee  ccoommppoouunnddss  iinn  aaqquuaaccuullttuurree

                                      E Evvaalluuaattiinngg  tthhee  aabbiilliittyy  ttoo  Plant extract  In vivo   Evaluating the ability of plant
                                                                             test
                                  • Enhance immunological                         extracts as immunostimulants
                                    parameters and growth      Mixing
                                    performance                       Feeding       and growth enhancer on
                                  • Enhance the resistance to                       striped catfish, red tilapia,
                                    pathogens in aquatic animals                      whiteleg shrimp and
                                                                 Fish feed  Challenging  snakehead were done
                                       D Deevveellooppiinngg  aa  pprroocceessss  ttoo  ssuupppplleemmeenntt
                                       p prroodduuccttss  wwiitthh  bbiiooaaccttiivvee  ccoommppoouunnddss  iinn    E Evvaalluuaattiinngg  tthhee  eeffffeeccttiivveenneessss  ooff  bbiiooaaccttiivvee
                                          f feeeedd  ffoorr  ssoommee  aaqquuaattiicc  aanniimmaallss  c coommppoouunndd  pprroodduuccttss  iinn  ffaarrmmss
                                                                    9 9//88 iinn  VViieettnnaammeessee    2 2//22  PPhhDD 9 9//1122  MMSScc
                                  Program outputs Program outputs  2 200    ppllaanntt  ssppeecciieess  &&    P Puubblliiccaattiioonnss  0 0//11 bbooookk  2 2//44 iinn    2 2//22  sshhoorrtt  ttrraaiinniinnggss
                                                                       j joouurrnnaall
                                        1 1 sscciieennttiiffiicc  rreeppoorrtt
                                                                                                1 166//1155  BBSScc
                                                                     i inntteerrnnaattiioonnaall  jjoouurrnnaall
                                        e exxttrraacctt  iiddeennttiiffiieedd
                                        2 2 ddeevveellooppmmeenntt  tteecchhnnoollooggyy    &&
                                                                                             0 0//6600  ttrraaiinneeee
                                        a apppplliiccaattiioonn  tteecchhnnoollooggyy
                6 Comments       This research still continues towards finalizing program outputs.
                ⓒ All Rights Reserved, Nov. 2020 (CTU)



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