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3 Progress of Joint Research Activities
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th
Research No.: ⑪ A-11 ODA Loan Joint Research Date: Oct. 12 , 2020
1 General Title Microbial and pharmaceutical studies
for animal disease treatment

2 Core CTU: Tran Ngoc BICH (Leader); Huynh Kim DIEU, Ly Thi Lien KHAI, Nguyen Loc HIEN, Nguyen Thu TAM, Bui Thi Le
Members MINH, Nguyen Phuc KHANH, Nguyen Thanh LAM, Nguyen Khanh THUAN, Chau Thi Huyen TRANG,
Nguyen Vinh TRUNG, Nguyen Thi Cam LOAN, Tran Duy KHANG
Japanese Universities: HAYASHIDANI Hideki (TUAT), TANIGUCHI Takahide (TUAT), SASSA Yukiko (TUAT)
3 Duration Oct. 2018 – Sep. 2021 (3 years) <2 Batch>
nd
4 Main To study strategies for prevention of microbial diseases and to develop pharmaceutical sources for
Objectives animal and poultry treatment

5 Focal Points Research topic 1: Infectious bronchitis infected in commercial chickens
IBV-VNTG6
IBV-VNTG3
HM194640.1_ ck/CH/LDL/091022
MN233837.1_CK/CH/SC/PZ/2017
82 JX840411.1_YX10
KY992864.1_VNUA8 QX-Like
GU111582.1_THA361052
100
MN262521.1_CK/CH/2014/FJ14
99 MG738155.1_IBS130/2015
MF924725.1_K2
100 FJ807946.1_KM91
KP790146.1_CK/CH/LHLJ/140901
77 100 HQ185567.1_CK/TW/T15/2006 Taiwan
95 DQ646405.2_TW2575/98
100 KM067901.1_M41-UPM2013
DQ830980.1_Massachusetts_41
100 IBV-VNVL11
100 IBV-VNST3
KJ435283.1_ck/CH/LSD/111219 Massachusetts (C)
AY561713.1_Ma5
98 FJ807652.1_H120
GQ885138.1_THA320352
EU817497.1_H52
AF286303.1_J2
70 (A) Phylogenetic analysis of nucleotide sequences of partial S1
100 AF227438.1_IBV Q1-Like
KY992863.1_VNUA3
100 AF286302.1_Q1
IBV-VNTG20 gene between detected IBV strains and references
U29523.1_N2/75
100 U29519.1_Vic_S Australia
KM067900.1_CR88-UPM2013 (B) Comparison of amino acid sequences of partial S protein
IBV-VNVL32
100 IBV-VNVL24
84 IBV-VNST13 793/B between detected IBV strains and references
KP118888.1_ck/CH/LLN/130102
KF377577.1_4/91_vaccine
99
AF093794.1_4/91_pathogenic
IBV-VNHG3 Hau Giang (C) Recombinant analysis of detected IBV strains
(A) (B)
IBV-VNHG17
MF176212.1_KrD1515
81
100
GQ265948.1_TC07-2
100 KY992865.1_VNUA11 TC07-2-Like
HQ018903.1_CK/CH/GD/NC10
Research topic 2: Prevalence and antimicrobial susceptibility of bacterial pathogens in domestic animals and
their products in the Mekong Delta
A
E E.. ccoollii oonn MMCC
B
The prevalence of pathogenic genes in Salmonella
isolated from domestic animals: sopE1(422 bp), spvC
(570 bp), and sopB (1170 bp)
M: DNA marker (100bp); P: positive control; N: C
negative control; well 1, 2: (+) sopE1; well 3, 4: (+)
spvC; well 5, 6: (+) sopB The genetic relationship of Salmonella isolated from domestic
animals, husbandry environment, and wild animals
Salmonella on BGA
Research topic 3: Medicinal plants in preventing and treating animal diseases
Electrophoresis spectrum of PCR products with OPB 07 primers on Agarose gel 1.5%
Electrophoresis spectrum of PCR products with OPE 14 primers on Agarose gel 1.5%
6 Perspectives This research still continues towards finalizing papers to submit Journals
ⓒ All Rights Reserved, Nov. 2020 (CTU)
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