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3 Progress of Joint Research Activities
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Research No.: ➉➉ A-10 ODA Loan Joint Research Date: Oct. 16 , 2020
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1 General Title Molecular studies for detection and identification of
animal diseases in Mekong Delta Region

2 Core Members CTU: Nguyen Trong Ngu (Leader), Luu Huu Manh, Nguyen Huu Hung, Ho Thi Viet Thu (Ms.),
Nguyen Nhut Xuan Dung (Ms.), Nguyen Thu Tam (Ms.), Bui Thi Le Minh (Ms.),
Huynh Ngoc Trang (Ms.), Nguyen Ho Bao Tran (Ms.), Pham Khanh Nguyen Huan, Huynh Tan Loc
Japanese Universities: KAMEI Kaeko (Ms.)(KIT), HAYASHIDANI Hideki (TUAT), TANIGUCHI Takahide (TUAT)
3 Duration Jan. 2018 – Dec. 2020 (3 years) <1 Batch>
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4 Main Objectives To apply molecular techniques for disease control systems involved all types of pathogenic agents
including bacteria, viruses, parasites and fungi for improving animal health
5 Preliminary results of workloads Outcomes of The experimental Effects of
The experimental experimental treatment of anticoccidial drugs
Research topic 1: Marek’s disease in chickens infection of chickens infection anticoccidial drugs and vaccines on the
with Eimeria species and vaccines Coccidia infection
The Coccidia infection rate rate
The prevalence of the Marek's disease virus (MDV) in chickens
serotype in some provinces of the Mekong Delta 100,00% The Coccidia infection
100,00 98,00% 30,00% rate in chickens
90,00 96,00%
25,00%
80,00 94,00% 20,00%
70,00 92,00% 15,00%
60,00
% 50,00 90,00% 10,00%
40,00 88,00% 5,00%
10^4 4 10 10 10^5 5
30,00 CFU/mL 0,00%
Control Vaccine Drug
20,00
10,00 Research topic 3: Mytotoxin in feed ingredients for chickens
0,00
Tra Vinh Hau Giang Vinh Long Long An Can Tho
The effect of the adsorbent materials (Hydrated The effect of the adsorbent materials (Biochar)
Serotype 1 Serotype 3 Serotype 1 & 3
minerals) on the aflatoxin content (ppb) on the aflatoxin content (ppb)
AFs AFs
G2 G2
Aflatoxin G1 Aflatoxin G1
B2 B2
B1 B1
0,0001 0,1001 0,2001 0,3001 0,4001 0,5001 0,6001 0,001 0,101 0,201 0,301 0,401 0,501
Phylogenetic tree of MDV strains isolated in the Mekong Delta Aflatoxin content (ppb) Aflatoxin content (ppb)
and reference strains. The numbers indicate BOOTSTRAP Kaolinte Bentonite Zeolite Hyacinth Rice husk Melaleuca Bamboo
coefficient values.
The efficiency of aflatoxin B1 adsorption: 99.17% The efficiency of aflatoxin B1 adsorption: 99.39%
(Zeolite), 99.51% (Bentonite), and 99.57% (Kaolinite) (Bamboo), 99.32% (Melaleuca), 99.27% (Rice husk) and,
Research topic 2: Coccidiosis in chickens 99.23% (Hyacinth)
Research topic 4: Probiotics and bacteriophages used for chickens
(a) (b)
Salmonella infections and treatment using Morphology of S11 (a) and S20 (b) bacteriophages
probiotics under STEM
Mortality rate of chickens
18
16
14
12
(c) (d) (e) 10 (a) (b)
%
8
6 Lesions in the internal organs of chicken infected with
4 Salmonella enterica subsp. enterica in the bacteriophage
2 therapy treatment
0
NT1 NT2 NT3 NT4 NT5 NT6 NT7
Treatment
NT1 (Control), NT2 (L. plantarum), NT3 (B. subtilis), NT4
Coccidioides species isolated from chickens in Ben Tre (Enprofloxacine), NT5 (Commercial product) NT6 (L. plantarum
province: (a) Eimeria acervulina, (b) Eimeria maxima, (c)
Eimeria mitis, (d) Eimeria necatrix, (e) Eimeria tenella. and B. subtilis), NT7 (Salmonella Typhimurium)
 7 national articles have been published.
6 Comments  01 patent applied.
 This research still continues towards finalizing papers to submit to international journals.
ⓒ All Rights Reserved, Nov. 2020 (CTU)
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